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W. D. Sam Solomon , Rahul A. Kumar , P. R. Vijai Anand , R. Sivakumar , R. Venkatnarayanan

Abstract

A selective, precise and derivatized HPTLC method has been developed for the simultaneous estimation of Glucosamine and Ibuprofen in tablet formulation. In this method standard and sample solutions of Glucosamine and Ibuprofen were applied on pre-coated silica gel 60F₂₅₄ TLC plate, and developed using Propanol-Ethyl acetate-Ammonia-Water (4: 3: 2: 1 v/v), as mobile phase and derivatized using Iodine vapor. The drugs on the plate were scanned at 254 nm. The dynamic linearity range was 5-25 μg/spot for Glucosamine and 2-10 μg/spot for Ibuprofen. The method was validated for precision, recovery and reproducibility.

Keywords

Simultaneous Estimation, HPTLC, Glucosamine and Ibuprofen.

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W. D. Sam Solomon ¹, Rahul A. Kumar ¹, P. R. Vijai Anand ¹, R. Venkatnarayanan ¹

Affiliations
1 Dept. of Pharmaceutical Analysis, RVS College of Pharmaceutical Sciences, Sulur, Coimbatore-641402, Tamilnadu, IN

Abstract

An accurate, precise and derivatized HPTLC method has been developed for the simultaneous estimation of Glucosamine, Vitamin C and Vitamin E in tablet formulation. In this method standard and sample solutions of Glucosamine, Vitamin C and Vitamin E were applied on pre-coated silica gel 60F₂₅₄ TLC plate, and developed using ethanol:Acetic acid (9:1 v/v), as mobile phase and derivatized using Iodine vapor. The drugs on the plate were scanned at 500 nm. The dynamic linearity range was 20-100 μg/spot for Glucosamine, 2-10 μg/spot for Vitamin C and 0.2-1 μg/spot for Vitamin E. The method was validated for precision, accuracy and reproducibility.

Keywords

Simultaneous Estimation, HPTLC, Glucosamine, Vitamin C and Vitamin E.

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